Although dendritic cell (DC) function is impaired in pancreatic cancer patients, the underlying mechanisms are unknown. This study analyzed the soluble factors released by pancreatic cancer cells responsible for inhibiting DC differentiation and activation. Medium conditioned by a highly metastatic human pancreatic cancer cell line BxPC-3 [BxPC-3 conditioned medium (BxCM)] was mainly used for ...
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Blood is a bodily fluid that contains abundant information about the health status of the individual. The average human adult has a blood volume of ∼5l continuously circulating throughout the body to deliver necessary nutrients and transport metabolic waste [1]. Blood consists of 54.3% plasma, 45% red blood cells (RBCs), and 0.7% white blood cells (WBCs) by volume [2]. Plasma is composed of ...
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QuantiGene Magnetic Separation Plates are 96-well flat-bottom plates used for the branched DNA and SAPE incubations on the second day of the QuantiGene Plex Assay. The Magnetic Separation Plates are compatible with the QuantiGene Handheld Magnetic Plate Washer (Cat. No. QP0702) and QuantiGene Plex P
Jun 23, 2020· HIV and EBV are human pathogens that cause a considerable burden to worldwide health. In combination, these viruses are linked to AIDS-associated lymphomas. We found that EBV, which transforms B cells, renders them susceptible to HIV-1 infection in a CXCR4 and CD4-dependent manner in vitro and that CXCR4-tropic HIV-1 integrates into the genome of these B cells with the …
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Jan 01, 2015· B cell autophagy is required for SLE induction in Tlr7.1 Tg mice. To study the role of autophagy in TLR7-mediated autoimmunity, we generated cohorts of WT and Tlr7.1 Tg mice with either intact or B cell-specific loss of autophagy (Cd19-Cre Atg5 f/f, referred to as Atg5 knockout [KO]) 24 (Table 1).For the majority of parameters tested in this paper Atg5 KO mice were indistinguishable from WT ...
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The QuantiGene Plex Assay Kit contains the generic reagents, buffers, and plates necessary to perform the assay in 96-well plates, and must be used with a target-specific QuantiGene Plex panel (sold separately). See related sample preparation reagents and RNA controls .The QuantiGene Plex Assay is a
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Nov 01, 2013· Tissue regeneration is a complex trait with few genetic models available. Mouse strains LG/J and MRL are exceptional healers. Using recombinant inbred strains from a large (LG/J, healer) and small (SM/J, nonhealer) intercross, we have previously shown a positive genetic correlation between ear wound healing, knee cartilage regeneration, and protection from osteoarthritis.
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Bio-Plex handheld magnetic washer For use with magnetic bead–based assays only Bio-Plex Pro flat bottom plates (40 x 96-well) For magnetic separation on the Bio-Plex Pro wash station Microtiter plate shaker IKA MTS 2/4 shaker for 2 or 4 microplates or Barnstead/Lab-Line Model 4625 plate
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E-waste management is a serious challenge across developed, transition, and developing countries because of the consumer society and the globalization process. E-waste is a fast-growing waste stream which needs more attention of international
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We studied whether allospecific CD4+ effector memory T cells (TEM) could induce graft-versus-host disease (GVHD) using a novel GVHD model induced solely by CD4+ T cell receptor transgenic TEa cells. Allospecific TEM generated in a lymphopenic host bore a typical memory phenotype. Moreover, these cells were able to elicit a faster and more effective proliferative response on challenge with ...
R^p 1 • Rep 3 Focfuction oflvfagrie Separation to Assay Sensitivity and specificity were insignificantly affected by time The BacTiter-GloTM assay reliably detected live E coli cells at D nee ntrat ions as low as 101 cfu/ml Although a slight decrease in sensitivity; a detection as low as 1.37x 103 cfu/ml remained possible with magnetic ...
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In this study, we show that RNA granules hitchhike on lysosomes for long-distance trafficking in mammalian cells. Using a combination of proximity labeling proteomics, live-cell imaging, and in vitro assays, we then identify the ALS-associated protein ANXA11 as a molecular tether that can dynamically couple RNA granules with lysosomes. ALS-associated mutations in ANXA11 disrupt docking between ...
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Jul 19, 2016· Magnetic separation was then performed to initially remove the non-γδ T cells. The TCR-γδ-positive T cells were then further bound with antibiotin magnetic microbeads. Separation was done using a magnetic separation column, and the γδ T cells were collected. The …
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Jan 19, 2018· A novel green synthesis strategy was proposed for preparation of multitemplate molecularly imprinted biopolymers (mt-MIBP) in aqueous media with less consumption of organic solvents, which were subsequently used as sorbents of ultrasound-assisted dispersive solid-phase extraction (d-SPE) for simultaneous recognition and efficient separation of B-family vitamins in juice …
Jun 17, 2014· Magnetic separator. 1. 1.1 Pengertian magnetic separation Magnetic Separation adalah suatu cara pemisahan/ konsentrasi dari mineral - mineral/ bijih berdasarkan sifat kemagnetan daripada mineral-mineral tersebut. Ketika mineral ditempatkan dalam medan magnet, ada tiga reaksi yang mungkin terjadi. Pertama,ada yang tertarik dengan medan magnet.